iLite® IL-12

Svar Life Science

iLite® IL-12

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Assay Ready Cells

Research Use Only

iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to IL-12 by specific and proportional expression of Firefly Luciferase. The iLite IL-12 assay ready cells can be used for the quantification IL-12 or p40 inhibitor activity, or for detecting a neutralizing antibody response against such inhibitors in human serum. The cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct under the control of a constitutive promotor. The assay ready iLite IL-12 cells are the perfect complement to the iLite® IL-23 Assay Ready Cells, allowing testing for specific IL-12 inhibitory activity and screening for unwanted inhibitory activity of IL-23. The ATCC® CRL-2111 cell line is used under a license from ATCC®

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