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Western Blot analysis of Raji and 231 cell, hepG2,hela and 293T cell using IMMT Polyclonal Antibody at dilution of 1:270
Western Blot analysis of Raji and 231 cell, hepG2,hela and 293T cell using IMMT Polyclonal Antibody at dilution of 1:270
Western Blot analysis of Raji and 231 cell, hepG2,hela and 293T cell using IMMT Polyclonal Antibody at dilution of 1:270

IMMT Polyclonal Antibody

E-AB-10395
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    IMMT Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    Mitochondrial inner membrane protein is a protein that in humans is encoded by the IMMT gene.IMMT has been shown to interact with BAT2.The mitochondrial inner membrane (IM) serves as the site for ATP production by hosting the oxidative phosphorylation complex machinery most notably on the crista membranes. Disruption of the crista structure has been implicated in a variety of cardiovascular and neurodegenerative diseases.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (A04102-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse HEPA1/6 whole cell lysates, Lane 7: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (Catalog # A04102-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.
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