Immunoglobulin G, Fab'2 Fragment

Athens Research

Immunoglobulin G, Fab'2 Fragment

9

Biotherapeutics, Flow Cytometry, Immunohistochemistry, Antisera Production, In Vitro Diagnostic

Research Use Only

≥95% by SDS-PAGE. Single arc by IEP against antisera to whole human serum.

The IgG F(ab')₂ fragment is a bivalent, proteolytic product of Immunoglobulin G, retaining two antigen-binding (Fab') regions connected by disulfide bonds in the hinge region while excluding the Fc domain2. This structural configuration preserves avidity through dual antigen engagement, offering a higher association constant compared to monovalent Fab fragments-particularly advantageous for low-affinity antibodies requiring enhanced binding stability. The absence of the Fc region eliminates interactions with Fc receptors and complement proteins, minimizing nonspecific immune activation and background noise in diagnostic assays3. Therapeutically, F(ab')₂ fragments are pivotal in antivenom formulations where rapid toxin neutralization without Fc-mediated inflammation reduces adverse reactions and mitigates late coagulopathy in rattlesnake envenomation. In oncology, their smaller size enhances tumor penetration, while faster blood clearance improves imaging signal-to-noise ratios in radiolabeled diagnostics Immunologically, Diagnostically, F(ab')₂ fragments enable precise antigen detection in flow cytometry and immunohistochemistry by avoiding Fc receptor cross-reactivity. Their divalent binding also stabilizes antigen-antibody complexes in immunoassays, ensuring high specificity for low-abundance targets. Emerging applications include autoimmune disease management, where F(ab')₂ fragments neutralize autoantibodies while circumventing complement activation. By combining targeted avidity with reduced immunogenicity, IgG F(ab')₂ fragments bridge therapeutic efficacy and diagnostic precision across diverse clinical contexts.

more then 1 year

Source human plasma non-reactive for HBsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

-20C

41116100