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IMP4 Antibody (aa102-281)

LS-C178526
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIMP4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IMP4 Antibody (aa102-281)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:20000 - 1:80000), IHC (1:100 - 1:500), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID92856
  • Target name
    IMP4
  • Target description
    IMP U3 small nucleolar ribonucleoprotein 4
  • Target synonyms
    BXDC4, U3 small nucleolar ribonucleoprotein IMP4, IMP4 homolog, U3 small nucleolar ribonucleoprotein, IMP4, U3 small nucleolar ribonucleoprotein, homolog, U3 small nucleolar ribonucleoprotein protein IMP4, U3 snoRNP protein 4 homolog, U3 snoRNP protein IMP4, brix domain-containing protein 4
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IMP4 using anti-IMP4 antibody (A10469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse EL-4 whole cell lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP4 antigen affinity purified polyclonal antibody (Catalog # A10469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP4 at approximately 36 kDa. The expected band size for IMP4 is at 34 kDa.
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