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IMPDH2 Antibody

LS-C401776
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIMPDH2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IMPDH2 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3615
  • Target name
    IMPDH2
  • Target description
    inosine monophosphate dehydrogenase 2
  • Target synonyms
    IMPD2, IMPDH-II, inosine-5'-monophosphate dehydrogenase 2, IMP (inosine 5'-monophosphate) dehydrogenase 2, IMP (inosine monophosphate) dehydrogenase 2, IMP dehydrogenase II, IMP oxireductase 2, IMPD 2, IMPDH 2, epididymis secretory sperm binding protein, inosine 5' phosphate dehydrogenase 2, inosine monophosphate dehydrogenase type II, inosine-5'-monophosphate dehydrogenase type II
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMPDH2 antigen affinity purified polyclonal antibody (Catalog # A03021-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMPDH2 at approximately 56 kDa. The expected band size for IMPDH2 is at 56 kDa.
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