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ProductsBack/Importin 9/RANBP9 Antibody (YA1625, Rabbit)

Importin 9/RANBP9 Antibody (YA1625, Rabbit)

HY-P81880
MedChem Express
TargetIPO9
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Importin 9/RANBP9 Antibody (YA1625, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID55705
  • Target name
    IPO9
  • Target description
    importin 9
  • Target synonyms
    Imp9, importin-9, ran-binding protein 9, ranBP9
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Importin 9/RANBP9 Antibody (YA1625) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1625), targeting Importin 9, with a predicted molecular weight of 116 kDa (observed band size: 116 kDa). Importin 9/RANBP9 Antibody (YA1625) can be used for WB, ICC/IF, IP experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IPO9 using anti-IPO9 antibody (A10216-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPO9 antigen affinity purified polyclonal antibody (Catalog # A10216-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPO9 at approximately 120 kDa. The expected band size for IPO9 is at 120 kDa.
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