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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA139485(IPO9 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA139485(IPO9 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA139485(IPO9 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

IPO9 Antibody

CSB-PA139485
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetIPO9
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Overview

  • Supplier
    Cusabio
  • Product Name
    IPO9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55705
  • Target name
    IPO9
  • Target description
    importin 9
  • Target synonyms
    Imp9, importin-9, ran-binding protein 9, ranBP9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96P70
  • Protein Name
    Importin-9
  • Scientific Description
    Importin-9, also known as Imp9 or IPO9, is a 1,041 amino acid protein that contains one importin N-terminal domain and belongs to the Importin- family. Localized to both the nucleus and the cytoplasm, Importin-9 functions as a nuclear transport receptor that mediates the docking of the Importin complex to the nuclear pore complex (NPC). Importin-9 mediates nuclear import of H2B histone, Ribosomal Protein S7 and Ribosomal Protein L18A. The gene encoding Importin-9 is located on human chromosome 1, the largest human chromosome that spans about 260 million base pairs and makes up 8% of the human genome.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IPO9 using anti-IPO9 antibody (A10216-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPO9 antigen affinity purified polyclonal antibody (Catalog # A10216-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPO9 at approximately 120 kDa. The expected band size for IPO9 is at 120 kDa.
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TargetIPO9
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TargetIPO9
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TargetIPO9
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