Assessment of the biotinylation efficiency by gel shift when testing BirA titration under different reaction conditions.
Assessment of the biotinylation efficiency by gel shift when testing BirA titration under different reaction conditions.
In Vitro Biotinylation Kit (with BirA tagless enzyme)
82511
Product group Molecular Biology
Overview
- SupplierBPS Bioscience
- Product NameIn Vitro Biotinylation Kit (with BirA tagless enzyme)
- Delivery Days Customer7
- Scientific DescriptionThe In Vitro Biotinylation Kit (with BirA Tagless Enzyme) is a kit designed for in vitro addition of biotin to biotin-acceptor peptides using BirA. This kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin. The BirA protein provided is a tagless recombinant biotin protein ligase (EC6.3.4.15). It efficiently catalyzes the specific attachment of biotin to the AviTag™. It is a robust reaction in a wide range of conditions, such as 20-40 microM of protein substrate (biotin-acceptor peptide) concentrations and can tolerate up to 1M NaCl and 20% glycerol in the reaction.
- Storage InstructionSee manual
- UNSPSC41116133