Influenza A virus Nucleoprotein antibody [HL1089] detects Influenza A virus Nucleoprotein protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded flu A_NP (Kansas/2017/H3N2) transfected 293T cell FFPE Cell Pellet Block. Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [HL1089] (GTX636318) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)](https://www.genetex.com/upload/website/prouct_img/normal/GTX636318/GTX636318_44389_20210927_LateralFlow_MD_InfluenzaA_w_23061202_179.webp "Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)")
![Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below.
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)
Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636318/GTX636318_T-44368_20210728_ELISA_PAIR_Viral_Multiple_w_23061202_179.webp "Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below.
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)
Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).
Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636247. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636318/GTX636318_T-44368_20210721_ELISA_PAIR_Multiple_3_w_23061202_682.webp "Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).
Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636247. Please contact us for custom HRP-conjugated antibody.")
![Influenza A viral lysate (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636318/GTX636318_T-44369_20210723_WB_InfluenzaA_w_23061202_316.webp "Influenza A viral lysate (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (GTX135903-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (20000-4.77*10 -3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 139.15 pM](https://www.genetex.com/upload/website/prouct_img/normal/GTX636318/GTX636318_T-44368_20210716_ELISA_Indirect_EC50_w_23061202_505.webp "Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (GTX135903-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (20000-4.77*10 -3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 139.15 pM")
![Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein protein (A/California/2009 (H1N1)), DDDDK Tag (GTX135904-pro) using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1098] (GTX636282) (5 μg/mL) Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (1 μg/mL) Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636282. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636318/GTX636318_T-44368_20210827_ELISA_PAIR_w_23061202_380.webp "Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein protein (A/California/2009 (H1N1)), DDDDK Tag (GTX135904-pro) using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1098] (GTX636282) (5 μg/mL) Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (1 μg/mL) Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636282. Please contact us for custom HRP-conjugated antibody.")
Influenza A virus Nucleoprotein antibody [HL1089] detects Influenza A virus Nucleoprotein protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded flu A_NP (Kansas/2017/H3N2) transfected 293T cell FFPE Cell Pellet Block. Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [HL1089] (GTX636318) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Influenza A virus Nucleoprotein antibody [HL1103]
GTX636318
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameInfluenza A virus Nucleoprotein antibody [HL1103]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1103
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Jiang X-M, Xin Q-L, Liu K, et al. Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner. mBio. 2023,14(6):e0168823. doi: 10.1128/mbio.01688-23Read this paper