Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
![Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_44347_20210723_WB_InfluenzaB_w_23061202_461.webp "Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_44347_20210730_ELISA_Indirect_Lysate_Multiple_Bar_w_23061202_414.webp "Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.")
![Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL. Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_T-44298_20210628_ELISA_PAIR_B_w_23061202_418.webp "Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL. Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_T-44298_20210730_ELISA_PAIR_Lysate_Multiple_w_23061202_248.webp "Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.")
![Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_44347_20210927_LateralFlow_MD_InfluenzaB_w_23061202_595.webp "Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)")
![Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_20210903_Immunoassay_MD_InfluenzaB_w_23061202_806.webp "Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)")
![Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636100/GTX636100_T-44298_20210728_ELISA_PAIR_Viral_Multiple_w_23061202_481.webp "Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.")
Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
Influenza B virus Nucleoprotein antibody [HL1069]
GTX636100
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameInfluenza B virus Nucleoprotein antibody [HL1069]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1069
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203