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ING1 Antibody (YA3018, Rabbit)

HY-P83273
MedChem Express
TargetING1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ING1 Antibody (YA3018, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3621
  • Target name
    ING1
  • Target description
    inhibitor of growth family member 1
  • Target synonyms
    p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a, inhibitor of growth protein 1, growth inhibitor ING1, growth inhibitory protein ING1, tumor suppressor ING1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ING1 Antibody (YA3018) is a non-conjugated IgG antibody, targeting ING1, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). ING1 Antibody (YA3018) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ING1 using anti-ING1 antibody (PB9644). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING1 antigen affinity purified polyclonal antibody (Catalog # PB9644) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING1 at approximately 47 kDa. The expected band size for ING1 is at 47 kDa.
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