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Immunohistochemistry of paraffin-embedded Human tonsil tissue using INHA Polyclonal Antibody at dilution 1:120
Immunohistochemistry of paraffin-embedded Human tonsil tissue using INHA Polyclonal Antibody at dilution 1:120
Immunohistochemistry of paraffin-embedded Human tonsil tissue using INHA Polyclonal Antibody at dilution 1:120

INHA Polyclonal Antibody

E-AB-10398
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetINHA
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Overview

  • Supplier
    Elabscience
  • Product Name
    INHA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3623
  • Target name
    INHA
  • Target description
    inhibin subunit alpha
  • Target synonyms
    inhibin alpha chain, A-inhibin subunit, inhibin alpha subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05111
  • Protein Name
    Inhibin alpha chain
  • Scientific Description
    This gene encodes the alpha subunit of inhibins A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.[
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of INHA using anti-INHA antibody (A02413-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse sp2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INHA antigen affinity purified polyclonal antibody (Catalog # A02413-3)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INHA at approximately 45KD. The expected band size for INHA is at 45KD.
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