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ICC/IF analysis of HeLa cells using GTX83223 Inhibin alpha antibody [4A2]. Green : Inhibin alpha Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of HeLa cells using GTX83223 Inhibin alpha antibody [4A2]. Green : Inhibin alpha Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of HeLa cells using GTX83223 Inhibin alpha antibody [4A2]. Green : Inhibin alpha Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments

Inhibin alpha antibody [4A2]

GTX83223
GeneTex
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetINHA
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Overview

  • Supplier
    GeneTex
  • Product Name
    Inhibin alpha antibody [4A2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4A2
  • Conjugate
    Unconjugated
  • Gene ID3623
  • Target name
    INHA
  • Target description
    inhibin subunit alpha
  • Target synonyms
    A-inhibin subunit; inhibin alpha chain; inhibin alpha subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05111
  • Protein Name
    Inhibin alpha chain
  • Scientific Description
    This gene encodes the alpha subunit of inhibins A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.[provided by RefSeq, Dec 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INHA using anti-INHA antibody (A02413-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse sp2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INHA antigen affinity purified polyclonal antibody (Catalog # A02413-3)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INHA at approximately 45KD. The expected band size for INHA is at 45KD.
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