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ProductsBack/Inorganic Pyrophosphatase 1 Recombinant Antbody Kit (KD-Validated)

Inorganic Pyrophosphatase 1 Recombinant Antbody Kit (KD-Validated)

BSM-90473R
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetPPA1
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Overview

  • Supplier
    Bioss
  • Product Name
    Inorganic Pyrophosphatase 1 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5464
  • Target name
    PPA1
  • Target description
    inorganic pyrophosphatase 1
  • Target synonyms
    HEL-S-66p, IOPPP, PP, PP1, SID6-8061, inorganic pyrophosphatase, PPase, cytosolic inorganic pyrophosphatase, diphosphate phosphohydrolase, epididymis secretory sperm binding protein Li 66p, inorganic diphosphatase 1, pyrophosphatase (inorganic) 1, pyrophosphatase 1, pyrophosphate phospho-hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15181
  • Protein Name
    Inorganic pyrophosphatase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA614884LA01HU diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of PPA1 using anti-PPA1 antibody (A07485-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPA1 antigen affinity purified polyclonal antibody (Catalog # A07485-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPA1 at approximately 33 kDa. The expected band size for PPA1 is at 33 kDa.
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