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ProductsBack/INPP4B Recombinant Antibody, Biotin Conjugated

INPP4B Recombinant Antibody, Biotin Conjugated

BSM-61303R-BIOTIN
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetINPP4B
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Overview

  • Supplier
    Bioss
  • Product Name
    INPP4B Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID8821
  • Target name
    INPP4B
  • Target description
    inositol polyphosphate-4-phosphatase type II B
  • Target synonyms
    inositol polyphosphate 4-phosphatase type II, inositol polyphosphate 4-phosphatase II; 4-phosphatase II, inositol polyphosphate-4-phosphatase, type II, 105kDa, type II inositol 3,4-bisphosphate 4-phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15327
  • Protein Name
    Inositol polyphosphate 4-phosphatase type II
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INPP4B using anti-INPP4B antibody (M06336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP4B antigen affinity purified monoclonal antibody (Catalog # M06336) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP4B at approximately 105 kDa. The expected band size for INPP4B is at 105,107 kDa.
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