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ProductsBack/INPP5D / SHIP1 / SHIP Antibody (C-Terminus)

INPP5D / SHIP1 / SHIP Antibody (C-Terminus)

LS-C368981
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetINPP5D
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    INPP5D / SHIP1 / SHIP Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3635
  • Target name
    INPP5D
  • Target description
    inositol polyphosphate-5-phosphatase D
  • Target synonyms
    SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, SH2 domain-containing inositol 5'-phosphatase 1, inositol polyphosphate-5-phosphatase, 145kD, inositol polyphosphate-5-phosphatase, 145kDa, phosphatidylinositol 4,5-bisphosphate 5-phosphatase, signaling inositol polyphosphate 5 phosphatase SIP-145, signaling inositol polyphosphate phosphatase SHIP II
  • Host
    Rabbit
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of INPP5D using anti-INPP5D antibody (A03358-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D antigen affinity purified polyclonal antibody (Catalog # A03358-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5D at approximately 145 kDa. The expected band size for INPP5D is at 133 kDa.
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