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INPPL1 Polyclonal Antibody

RD80239A
Reddot Biotech
ApplicationsWestern Blot
Product group Antibodies
TargetINPPL1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    INPPL1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3636
  • Target name
    INPPL1
  • Target description
    inositol polyphosphate phosphatase like 1
  • Target synonyms
    51C protein; INPPL-1; OPSMD; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2; protein 51C; SH2 domain-containing inositol-5'-phosphatase 2; SHIP2; SHIP-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene is an SH2-containing 5-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INPPL1 using anti-INPPL1 antibody (A01790). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPPL1 antigen affinity purified polyclonal antibody (Catalog # A01790) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPPL1 at approximately 150 kDa. The expected band size for INPPL1 is at 139 kDa.
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