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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: INSIG1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 18 kDa Observed band size: 46 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: INSIG1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 18 kDa Observed band size: 46 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: INSIG1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 18 kDa Observed band size: 46 kDa

INSIG1 Antibody

CSB-PA011745LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetINSIG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    INSIG1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3638
  • Target name
    INSIG1
  • Target description
    insulin induced gene 1
  • Target synonyms
    CL6, insulin-induced gene 1 protein, INSIG-1 membrane protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15503
  • Protein Name
    Insulin-induced gene 1 protein
  • Scientific Description
    Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of INSIG1 using anti-INSIG1 antibody (A02026-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HUH7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INSIG1 antigen affinity purified polyclonal antibody (Catalog # A02026-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INSIG1 at approximately 66 kDa. The expected band size for INSIG1 is at 30 kDa.
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