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ProductsBack/Insulin Receptor Substrate 1 (IRS1) Polyclonal Antibody

Insulin Receptor Substrate 1 (IRS1) Polyclonal Antibody

CAU24144
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetIrs1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Insulin Receptor Substrate 1 (IRS1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16367
  • Target name
    Irs1
  • Target description
    insulin receptor substrate 1
  • Target synonyms
    G972; G972R; insulin receptor substrate 1; IR; IRS-1
  • Host
    Rabbit
  • Protein IDP35569
  • Protein Name
    Insulin receptor substrate 1
  • Scientific Description
    The Insulin Receptor Substrate 1 (IRS1) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1(Ser789) Polyclonal Antibody(bs-3197R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Anti-IRS1 antibody, PA2269, All Western blotting All lanes: Anti-IRS1(PA2269) at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ug Lane 2: A549 Whole Cell Lysate at 40ug Lane 3: 293T Whole Cell Lysate at 40ug Lane 4: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 132KD Observed bind size: 132KD
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WB analysis of whole cell extracts (30 microg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), Jurkat (lane 4), HEK-293 (lane 5) and Panc-1 (lane 6) using GTX25602 IRS1 antibody. Dilution : 1:250
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References
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