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ProductsBack/Integrin alpha 4/CD49D Antibody (YA3201, Rabbit)

Integrin alpha 4/CD49D Antibody (YA3201, Rabbit)

HY-P83456
MedChem Express
TargetITGA4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Integrin alpha 4/CD49D Antibody (YA3201, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3676
  • Target name
    ITGA4
  • Target description
    integrin subunit alpha 4
  • Target synonyms
    CD49D, IA4, integrin alpha-4, 269C wild type, CD49 antigen-like family member D, VLA-4 subunit alpha, alpha 4 subunit of VLA-4 receptor, antigen CD49D, alpha-4 subunit of VLA-4 receptor, integrin alpha-IV, very late activation protein 4 receptor, alpha 4 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Integrin alpha 4/CD49D Antibody (YA3201) is a non-conjugated IgG antibody, targeting Integrin alpha 4, with a predicted molecular weight of 115 kDa (observed band size: 150 kDa). Integrin alpha 4/CD49D Antibody (YA3201) can be used for WB experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody (PB9648). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: JURKAT Whole Cell Lysate at 40ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: HEPG2 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 4 antigen affinity purified polyclonal antibody (Catalog # PB9648) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 4 at approximately 220 kDa. The expected band size for Integrin alpha 4 is at 115 kDa.
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