Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with GTX25190 Integrin beta 3 (phospho Tyr773) antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.
Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with GTX25190 Integrin beta 3 (phospho Tyr773) antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.
Integrin beta 3 (phospho Tyr773) antibody
GTX25190
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityFeline, Human
TargetITGB3
Overview
- SupplierGeneTex
- Product NameIntegrin beta 3 (phospho Tyr773) antibody
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID3690
- Target nameITGB3
- Target descriptionintegrin subunit beta 3
- Target synonymsantigen CD61; BDPLT16; BDPLT2; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2; integrin beta 3; integrin beta chain, beta 3; integrin beta-3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); platelet membrane glycoprotein IIIa
- HostRabbit
- IsotypeIgG
- Protein IDP05106
- Protein NameIntegrin beta-3
- Scientific DescriptionThe ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
- ReactivityFeline, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203