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Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated (bs-0342R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated (bs-0342R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated (bs-0342R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

ITGB3 Polyclonal Antibody

BS-0342R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetITGB3
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Overview

  • Supplier
    Bioss
  • Product Name
    ITGB3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3690
  • Target name
    ITGB3
  • Target description
    integrin subunit beta 3
  • Target synonyms
    BDPLT16, BDPLT2, BDPLT24, CD61, FMAIT1, GP3A, GPIIIa, GT, GT2, integrin beta-3, antigen CD61, integrin beta 3, integrin beta chain, beta 3, integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61), platelet membrane glycoprotein IIIa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05106
  • Protein Name
    Integrin beta-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chi Y, He P, Lei L, et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. Mol Med Rep. 2018,17(5):6337-6344. doi: 10.3892/mmr.2018.8685
    Read this paper
  • Qu XL, Ming-Zhang, Yuan-Fang, et al. Effect of 2,3',4,4',5-Pentachlorobiphenyl Exposure on Endometrial Receptivity and the Methylation of HOXA10. Reprod Sci. 2018,25(2):256-268. doi: 10.1177/1933719117711258
    Read this paper
  • Zhang L, Erfle H, Harder N, et al. High-Throughput RNAi Screening Identifies a Role for the Osteopontin Pathway in Proliferation and Migration of Human Aortic Smooth Muscle Cells. Cardiovasc Drugs Ther. 2016,30(3):281-95. doi: 10.1007/s10557-016-6663-4
    Read this paper
  • Li F, Liu Y, Kan X, et al. Elevated expression of integrin αv and β5 subunit in laryngeal squamous-cell carcinoma associated with lymphatic metastasis and angiogenesis. Pathol Res Pract. 2013,209(2):105-9. doi: 10.1016/j.prp.2012.11.001
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 3/ITGB3 antigen affinity purified polyclonal antibody (Catalog # A00587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 3/ITGB3 at approximately 100 kDa. The expected band size for Integrin beta 3/ITGB3 is at 87 kDa.
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