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ProductsBack/Interferon gamma antibody
WB analysis of various samples using GTX11759 Interferon gamma antibody. Lane 1 : Recombinant human IFN gamma protein 10ng Lane 2 : Recombinant human IFN gamma protein 5ng Lane 3 : Recombinant human IFN gamma protein 2.5ng Lane 4 : Recombinant human IFN gamma protein 1.25ng Dilution : 0.5 μg/mL
WB analysis of various samples using GTX11759 Interferon gamma antibody. Lane 1 : Recombinant human IFN gamma protein 10ng Lane 2 : Recombinant human IFN gamma protein 5ng Lane 3 : Recombinant human IFN gamma protein 2.5ng Lane 4 : Recombinant human IFN gamma protein 1.25ng Dilution : 0.5 μg/mL
WB analysis of various samples using GTX11759 Interferon gamma antibody. Lane 1 : Recombinant human IFN gamma protein 10ng Lane 2 : Recombinant human IFN gamma protein 5ng Lane 3 : Recombinant human IFN gamma protein 2.5ng Lane 4 : Recombinant human IFN gamma protein 1.25ng Dilution : 0.5 μg/mL

Interferon gamma antibody

GTX11759
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetIFNG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Interferon gamma antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID3458
  • Target name
    IFNG
  • Target description
    interferon gamma
  • Target synonyms
    IFG, IFI, IMD69, interferon gamma, IFN-gamma, immune interferon
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01579
  • Protein Name
    Interferon gamma
  • Scientific Description
    This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19 kDa. The expected band size for IFNG is at 19 kDa.
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