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Interleukin 16 (IL16) Polyclonal Antibody

CAU28780
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetIl16
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Overview

  • Supplier
    Biomatik
  • Product Name
    Interleukin 16 (IL16) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.94 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16170
  • Target name
    Il16
  • Target description
    interleukin 16
  • Target synonyms
    mKIAA4048, pro-interleukin-16
  • Host
    Rabbit
  • Protein IDO54824
  • Protein Name
    Pro-interleukin-16
  • Scientific Description
    The Interleukin 16 (IL16) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-16 using anti-IL-16 antibody (A01635). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse IL-16 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-16 antigen affinity purified polyclonal antibody (Catalog # A01635) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-16 at approximately 18KD. The expected band size for IL-16 is at 13KD.
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ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIl16
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