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Intrinsic Factor Polyclonal Antibody

BS-18173R
Bioss Antibodies
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCBLIF
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Intrinsic Factor Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2694
  • Target name
    CBLIF
  • Target description
    cobalamin binding intrinsic factor
  • Target synonyms
    cobalamin binding intrinsic factor; gastric intrinsic factor (vitamin B synthesis); GIF; IF; IFMH; INF; intrinsic factor; TCN3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27352
  • Protein Name
    Gastric intrinsic factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBLIF using anti-CBLIF antibody (A00038-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HGC-27 whole cell lysates, Lane 2: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBLIF antigen affinity purified polyclonal antibody (Catalog # A00038-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBLIF at approximately 52 kDa. The expected band size for CBLIF is at 45 kDa.
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