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ProductsBack/INTS4 antibody [N1N2], N-term
Sample (30 ug of whole cell lysate) A: U87-MG B: MCF-7 7.5% SDS PAGE GTX116519 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: U87-MG B: MCF-7 7.5% SDS PAGE GTX116519 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: U87-MG B: MCF-7 7.5% SDS PAGE GTX116519 diluted at 1:1000

INTS4 antibody [N1N2], N-term

GTX116519
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetINTS4
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Overview

  • Supplier
    GeneTex
  • Product Name
    INTS4 antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID92105
  • Target name
    INTS4
  • Target description
    integrator complex subunit 4
  • Target synonyms
    INT4, MST093, integrator complex subunit 4, MSTP093
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96HW7
  • Protein Name
    Integrator complex subunit 4
  • Scientific Description
    INTS4 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INTS4 using anti-INTS4 antibody (A13277-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS4 antigen affinity purified polyclonal antibody (Catalog # A13277-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTS4 at approximately 140 kDa. The expected band size for INTS4 is at 108 kDa.
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