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The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA547383(IPO8 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA547383(IPO8 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA547383(IPO8 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

IPO8 Antibody

CSB-PA547383
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIPO8
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Overview

  • Supplier
    Cusabio
  • Product Name
    IPO8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10526
  • Target name
    IPO8
  • Target description
    importin 8
  • Target synonyms
    imp8; importin-8; RAN binding protein 8; ran-binding protein 8; RANBP8; VISS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15397
  • Protein Name
    Importin-8
  • Scientific Description
    The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Importin 8 Polyclonal Antibody, Unconjugated (bs-20110R) at 1:200 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of IPO8 using anti-IPO8 antibody (A07327-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPO8 antigen affinity purified polyclonal antibody (Catalog # A07327-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPO8 at approximately 120 kDa. The expected band size for IPO8 is at 120 kDa.
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