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Western Blot Positive WB detected in: Rat kidney tissue All lanes: IPP antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: IPP antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: IPP antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa

IPP Antibody

CSB-PA011782LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetIPP
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Overview

  • Supplier
    Cusabio
  • Product Name
    IPP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3652
  • Target name
    IPP
  • Target description
    intracisternal A particle-promoted polypeptide
  • Target synonyms
    KLHL27, actin-binding protein IPP, kelch-like family member 27, kelch-like protein 27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y573
  • Protein Name
    Actin-binding protein IPP
  • Scientific Description
    May play a role in organizing the actin cytoskeleton.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IPP using anti-IPP antibody (A07236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPP antigen affinity purified polyclonal antibody (Catalog # A07236-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPP at approximately 65 kDa. The expected band size for IPP is at 65 kDa.
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