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IQGAP2 Recombinant Antbody Kit (KD-Validated)

BSM-90011R
Bioss Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetIQGAP2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IQGAP2 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID10788
  • Target name
    IQGAP2
  • Target description
    IQ motif containing GTPase activating protein 2
  • Target synonyms
    ras GTPase-activating-like protein IQGAP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13576
  • Protein Name
    Ras GTPase-activating-like protein IQGAP2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IQGAP2 using anti-IQGAP2 antibody (A04787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 cell lysates, Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP2 antigen affinity purified polyclonal antibody (Catalog # A04787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP2 at approximately 181KD. The expected band size for IQGAP2 is at 181KD.
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