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IRAK4 / IRAK-4 Antibody

LS-C812660
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIRAK4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IRAK4 / IRAK-4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IHC (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51135
  • Target name
    IRAK4
  • Target description
    interleukin 1 receptor associated kinase 4
  • Target synonyms
    IMD67; interleukin-1 receptor-associated kinase 4; IPD1; IRAK-4; NY-REN-64; REN64; renal carcinoma antigen NY-REN-64
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Muscle lysates; Lane 2: Mouse Stomach lysates; Lane 3: Rat Muscle lysates probed with IRAK4 Polyclonal Antibody, Unconjugated (bs-2440R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of IRAK4 using anti-IRAK4 antibody (A01247-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK4 antigen affinity purified polyclonal antibody (Catalog # A01247-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRAK4 at approximately 52 kDa. The expected band size for IRAK4 is at 52 kDa.
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