Paraformaldehyde-fixed, paraffin embedded Rat brainy; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRE1a (Ser726) Polyclonal Antibody, Unconjugated (bs-4308R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brainy; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRE1a (Ser726) Polyclonal Antibody, Unconjugated (bs-4308R) at 1:400 overnight at 4°C, DAB staining.
phospho-IRE1a (Ser 726) Polyclonal Antibody
BS-4308R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetERN1
Overview
- SupplierBioss
- Product Namephospho-IRE1a (Ser 726) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2081
- Target nameERN1
- Target descriptionendoplasmic reticulum to nucleus signaling 1
- Target synonymsIRE1, IRE1P, IRE1a, hIRE1p, serine/threonine-protein kinase/endoribonuclease IRE1, ER to nucleus signalling 1, inositol-requiring 1, inositol-requiring enzyme 1, inositol-requiring protein 1, ire1-alpha, protein kinase/endoribonuclease
- HostRabbit
- IsotypeIgG
- Protein IDO75460
- Protein NameSerine/threonine-protein kinase/endoribonuclease IRE1
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC41116161
References
- Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Abeta toxicity via attenuating Abeta-induced endoplasmic reticulum stress. Xu S et al., 2019 Feb 13, J NeuroinflammationRead this paper
- Vesicular nucleotide transporter mediates ATP release and migration in neutrophils. Harada Y et al., 2018 Mar 9, J Biol ChemRead this paper
- Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Li L et al., 2018 Mar 15, Toxicol LettRead this paper
Datasheet
Related products
Product group Antibodies
ERN1 AntibodyCSB-PA007795LA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetERN1
- SizePrice
Product group Antibodies
Anti-IRE1 AntibodyA92812
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
- SizePrice
Product group Antibodies
Goat anti-ERN1 / IRE1aEB08559
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetERN1
- SizePrice
Product group Antibodies
Anti-ERN1 AntibodyHPA027730
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetERN1
- SizePrice
Product group Antibodies
ERN1 / IRE1 Antibody (FITC)LS-C498811
ApplicationsELISA
ReactivityHuman
TargetERN1
- SizePrice
Product group Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetERN1
- SizePrice
Product group Antibodies
References
ApplicationsWestern Blot
ReactivityHuman
TargetERN1
- SizePrice
![IRE1 alpha antibody [N1N2], N-term detects IRE1 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IRE1 alpha stained by IRE1 alpha antibody [N1N2], N-term (GTX113682) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX113682/GTX113682_40142_20200522_IHC-P_R_w_23060501_806.webp)
Product group Antibodies
IRE1 alpha antibody [N1N2], N-termGTX113682
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Rat
TargetERN1
- SizePrice