Paraformaldehyde-fixed, paraffin embedded Rat brainy; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRE1a (Ser726) Polyclonal Antibody, Unconjugated (bs-4308R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brainy; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRE1a (Ser726) Polyclonal Antibody, Unconjugated (bs-4308R) at 1:400 overnight at 4°C, DAB staining.
phospho-IRE1a (Ser 726) Polyclonal Antibody
BS-4308R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetERN1
Overview
- SupplierBioss
- Product Namephospho-IRE1a (Ser 726) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2081
- Target nameERN1
- Target descriptionendoplasmic reticulum to nucleus signaling 1
- Target synonymsIRE1, IRE1P, IRE1a, hIRE1p, serine/threonine-protein kinase/endoribonuclease IRE1, ER to nucleus signalling 1, inositol-requiring 1, inositol-requiring enzyme 1, inositol-requiring protein 1, ire1-alpha, protein kinase/endoribonuclease
- HostRabbit
- IsotypeIgG
- Protein IDO75460
- Protein NameSerine/threonine-protein kinase/endoribonuclease IRE1
- Storage Instruction-20°C
- UNSPSC12352203
References
- Xu S, Di Z, He Y, et al. Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stress. J Neuroinflammation. 2019,16(1):35. doi: 10.1186/s12974-019-1429-0Read this paper
- Harada Y, Kato Y, Miyaji T, et al. Vesicular nucleotide transporter mediates ATP release and migration in neutrophils. J Biol Chem. 2018,293(10):3770-3779. doi: 10.1074/jbc.M117.810168Read this paper
- Li L, Cui J, Liu Z, et al. Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Toxicol Lett. 2018,285:156-167. doi: 10.1016/j.toxlet.2018.01.004Read this paper
Datasheet
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