Paraformaldehyde-fixed, paraffin embedded Rat brainy; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRE1a (Ser726) Polyclonal Antibody, Unconjugated (bs-4308R) at 1:400 overnight at 4°C, DAB staining.
IRE1a (Ser726) Polyclonal Antibody
BS-4308R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetERN1
Overview
- SupplierBioss Antibodies
- Product NameIRE1a (Ser726) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2081
- Target nameERN1
- Target descriptionendoplasmic reticulum to nucleus signaling 1
- Target synonymsER to nucleus signalling 1; hIRE1p; inositol-requiring 1; inositol-requiring enzyme 1; inositol-requiring protein 1; IRE1; IRE1a; ire1-alpha; IRE1P; protein kinase/endoribonuclease; serine/threonine-protein kinase/endoribonuclease IRE1
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDO75460
- Protein NameSerine/threonine-protein kinase/endoribonuclease IRE1
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Abeta toxicity via attenuating Abeta-induced endoplasmic reticulum stress. Xu S et al., 2019 Feb 13, J NeuroinflammationRead more
- Vesicular nucleotide transporter mediates ATP release and migration in neutrophils. Harada Y et al., 2018 Mar 9, J Biol ChemRead more
- Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Li L et al., 2018 Mar 15, Toxicol LettRead more