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ICC/IF analysis of HeLa cells using GTX03422 IRF2 antibody. Blue : DAPI for nuclear staining
ICC/IF analysis of HeLa cells using GTX03422 IRF2 antibody. Blue : DAPI for nuclear staining
ICC/IF analysis of HeLa cells using GTX03422 IRF2 antibody. Blue : DAPI for nuclear staining

IRF2 antibody

GTX03422
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetIRF2
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Overview

  • Supplier
    GeneTex
  • Product Name
    IRF2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3660
  • Target name
    IRF2
  • Target description
    interferon regulatory factor 2
  • Target synonyms
    interferon regulatory factor 2; IRF-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRF2 using anti-IRF2 antibody (PB9645). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Intestine Tissue Lysate at 50ug, Lane 2: SW620 Whole Cell Lysate at 40ug, Lane 3: COLO320 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2 antigen affinity purified polyclonal antibody (Catalog # PB9645) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF2 at approximately 50 kDa. The expected band size for IRF2 is at 39 kDa.
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