Cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IRF3 antibody [3F10]
GTX50058
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetIRF3
Overview
- SupplierGeneTex
- Product NameIRF3 antibody [3F10]
- Delivery Days Customer9
- Application Supplier NoteWe recommend the following starting dilutions:Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3F10
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3661
- Target nameIRF3
- Target descriptioninterferon regulatory factor 3
- Target synonymsIIAE7, interferon regulatory factor 3
- HostMouse
- IsotypeIgG1
- Scientific DescriptionThis gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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