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Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, SH-SY5Y whole cell lysate All lanes: IRF5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 58, 55, 48, 18 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, SH-SY5Y whole cell lysate All lanes: IRF5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 58, 55, 48, 18 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, SH-SY5Y whole cell lysate All lanes: IRF5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 58, 55, 48, 18 kDa Observed band size: 57 kDa

IRF5 Antibody

CSB-PA011820LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIRF5
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Overview

  • Supplier
    Cusabio
  • Product Name
    IRF5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3663
  • Target name
    IRF5
  • Target description
    interferon regulatory factor 5
  • Target synonyms
    interferon regulatory factor 5; SLEB10
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRF5 using anti-IRF5 antibody (A00958-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 antigen affinity purified polyclonal antibody (Catalog # A00958-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF5 at approximately 60 kDa. The expected band size for IRF5 is at 56 kDa.
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