ICC/IF analysis of T-47D cells using GTX25604 IRS1 antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton X-100 for 10 minutes Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature
ICC/IF analysis of T-47D cells using GTX25604 IRS1 antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton X-100 for 10 minutes Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature
IRS1 antibody
GTX25604
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetIrs1
Overview
- SupplierGeneTex
- Product NameIRS1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 2 microg/ml. ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID16367
- Target nameIrs1
- Target descriptioninsulin receptor substrate 1
- Target synonymsG972; G972R; insulin receptor substrate 1; IR; IRS-1
- HostRabbit
- IsotypeIgG
- Protein IDP35569
- Protein NameInsulin receptor substrate 1
- Scientific DescriptionInsulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilms tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option. To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203