Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU(GTX115709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![ISCU antibody [N1C3] detects ISCU protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ISCU stained by ISCU antibody [N1C3] (GTX115709) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115709/GTX115709_43104_20180307_ICC_IF_w_23060519_116.webp "ISCU antibody [N1C3] detects ISCU protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ISCU stained by ISCU antibody [N1C3] (GTX115709) diluted at 1:500. Blue: Hoechst 33342 staining.")
![ISCU antibody [N1C3] detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ISCU antibody [N1C3] (GTX115709) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX115709/GTX115709_40296_IHC_M_w_23060519_174.webp "ISCU antibody [N1C3] detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ISCU antibody [N1C3] (GTX115709) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] (GTX115709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115709/GTX115709_43104_20180216_WB_B_w_23060519_126.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] (GTX115709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] (GTX115709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115709/GTX115709_43104_20180216_WB_w_23060519_869.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] (GTX115709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU(GTX115709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
ISCU antibody [N1C3]
GTX115709
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetISCU
Overview
- SupplierGeneTex
- Product NameISCU antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.66 mg/ml
- ConjugateUnconjugated
- Gene ID23479
- Target nameISCU
- Target descriptioniron-sulfur cluster assembly enzyme
- Target synonyms2310020H20Rik, HML, ISU2, NIFU, NIFUN, hnifU, iron-sulfur cluster assembly enzyme ISCU, IscU iron-sulfur cluster scaffold homolog, iron-sulfur cluster assembly enzyme ISCU, mitochondrial, nifU-like N-terminal domain-containing protein
- HostRabbit
- IsotypeIgG
- Protein IDQ9H1K1
- Protein NameIron-sulfur cluster assembly enzyme ISCU
- Scientific DescriptionIron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chen C, Wang S, Liu P. Deferoxamine Enhanced Mitochondrial Iron Accumulation and Promoted Cell Migration in Triple-Negative MDA-MB-231 Breast Cancer Cells Via a ROS-Dependent Mechanism. Int J Mol Sci. 2019,20(19). doi: 10.3390/ijms20194952Read this paper
Datasheet
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