ISG15 (human) (rec.) (Rhodamine 110)

South Bay Bio

ISG15 (human) (rec.) (Rhodamine 110)

10

Research Use Only

TRITC

>97%

Liquid

Ubiquitin-like protein ISG15

ISG15 (Interferon Stimulated Gene, 15kDa) is a ubiquitin-like modifier which initiates innate immune response by activating RIG-I signaling, stimulating NK-cell proliferation, inhibiting viral budding and acting as an IFNgamma-inducing cytokine. ISG15 contains two tandem ubiquitin homology domains and is cross-reactive with alpha-Ubiquitin antibodies. Conjugation of ISG15 to a substrate protein occurs through a ubiquitin like cascade via an E1 (UBE1L), E2 (UbcH8/UBE2L6) and E3 (not yet discovered.) Deconjugation normally occurs via UBP43 (USP18), however there are several viral proteases that are able to hydrolyze ISG15 conjugates in order to evade immune response. These include the OTU-containing protease of Crimean-Congo Hemorrhagic Fever nairovirus and the Papain-Like Protease (PLPro) of the SARS coronavirus. - Protein. Human ISG15 (aa1-157)conjugated at the C-terminus to a quenched Rhodamine 110 dye. Source: E. coli. Formulation: Liquid. In 50mM MES pH 6.0, 100mM sodium chloride, 10% glycerol. Purity: >97% (LCMS). ISG15 (Interferon Stimulated Gene, 15kDa) is a ubiquitin-like modifier which initiates innate immune response by activating RIG-I signaling, stimulating NK-cell proliferation, inhibiting viral budding and acting as an IFNgamma-inducing cytokine. ISG15 contains two tandem ubiquitin homology domains and is cross-reactive with alpha-Ubiquitin antibodies. Conjugation of ISG15 to a substrate protein occurs through a ubiquitin like cascade via an E1 (UBE1L), E2 (UbcH8/UBE2L6) and E3 (not yet discovered.) Deconjugation normally occurs via UBP43 (USP18), however there are several viral proteases that are able to hydrolyze ISG15 conjugates in order to evade immune response. These include the OTU-containing protease of Crimean-Congo Hemorrhagic Fever nairovirus and the Papain-Like Protease (PLPro) of the SARS coronavirus.

-80°C

12352202