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ProductsBack/ISGF-3gamma p48 (H-10) Alexa Fluor(r) 546

ISGF-3gamma p48 (H-10) Alexa Fluor(r) 546

SC-365893AF546
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIRF9
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    ISGF-3gamma p48 (H-10) Alexa Fluor(r) 546
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 546
  • Gene ID10379
  • Target name
    IRF9
  • Target description
    interferon regulatory factor 9
  • Target synonyms
    IRF-9, ISGF3, ISGF3G, p48, interferon regulatory factor 9, IFN-alpha-responsive transcription factor subunit, ISGF-3 gamma, ISGF3 p48 subunit, interferon-stimulated gene factor 3 gamma, interferon-stimulated transcription factor 3, gamma (48kD), interferon-stimulated transcription factor 3, gamma 48kDa, transcriptional regulator ISGF3 subunit gamma
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRF9 using anti-IRF9 antibody (A04485). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: JURKAT cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF9 antigen affinity purified polyclonal antibody (Catalog # A04485) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF9 at approximately 44KD. The expected band size for IRF9 is at 44KD.
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