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Isocitrate Assay Kit (Fluorometric)

LS-K219
LifeSpan BioSciences
Product group Assays
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Isocitrate Assay Kit (Fluorometric)
  • Delivery Days Customer
    14
  • Application Supplier Note
    For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
  • Applications Supplier
    Spectrophotometry (fluorometric) For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
  • Estimated Purity
    ...
  • Scientific Description
    ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing Alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. This isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116133