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IST1 Antibody, Biotin conjugated

MBS7107942
MyBioSource
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Product group Antibodies
Price on request
0.05 mg
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Overview

  • Supplier
    MyBioSource
  • Product Name
    IST1 Antibody, Biotin conjugated
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Estimated Purity
    >95%, Protein G purified
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MRC-5 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1/IST1 antigen affinity purified polyclonal antibody (Catalog # A05507-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1/IST1 at approximately 40 kDa. The expected band size for HIST1/IST1 is at 40 kDa.
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