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IST1 antibody

GTX101972
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetIST1
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Overview

  • Supplier
    GeneTex
  • Product Name
    IST1 antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9798
  • Target name
    IST1
  • Target description
    IST1 factor associated with ESCRT-III
  • Target synonyms
    CHMP8, OLC1, IST1 homolog, IST1, ESCRT-III associated factor, IST1, endosomal sorting complex required for transport-III component, charged multivesicular body protein 8, increased sodium tolerance 1 homolog, overexpressed in lung cancer 1, putative MAPK-activating protein PM28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53990
  • Protein Name
    IST1 homolog
  • Scientific Description
    Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Varland S, Silva RD, Kjosås I, et al. N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity. Nat Commun. 2023,14(1):6774. doi: 10.1038/s41467-023-42342-y
    Read this paper
  • Chang CL, Weigel AV, Ioannou MS, et al. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. J Cell Biol. 2019,218(8):2583-2599. doi: 10.1083/jcb.201902061
    Read this paper
  • Feng Q, Luo Y, Zhang XN, et al. MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration. Autophagy. 2020,16(4):641-658. doi: 10.1080/15548627.2019.1633862
    Read this paper
  • Renvoisé B, Stadler J, Singh R, et al. Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling. Hum Mol Genet. 2012,21(16):3604-18. doi: 10.1093/hmg/dds191
    Read this paper

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Figure 1. Western blot analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MRC-5 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1/IST1 antigen affinity purified polyclonal antibody (Catalog # A05507-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1/IST1 at approximately 40 kDa. The expected band size for HIST1/IST1 is at 40 kDa.
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