More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IST1 antibody

IST1 antibody

GTX101972
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetIST1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    IST1 antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9798
  • Target name
    IST1
  • Target description
    IST1 factor associated with ESCRT-III
  • Target synonyms
    CHMP8, OLC1, IST1 homolog, IST1, ESCRT-III associated factor, IST1, endosomal sorting complex required for transport-III component, charged multivesicular body protein 8, increased sodium tolerance 1 homolog, overexpressed in lung cancer 1, putative MAPK-activating protein PM28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53990
  • Protein Name
    IST1 homolog
  • Scientific Description
    Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity.
    Read more
  • Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. Chang CL et al., 2019 Aug 5, J Cell Biol
    Read more
  • MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration. Feng Q et al., 2020 Apr, Autophagy
    Read more
  • Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling. Renvoise B et al., 2012 Aug 15, Hum Mol Genet
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

IHC image of CSB-PA012171LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
IST1 AntibodyCSB-PA012171LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIST1
  • SizePrice
WB analysis of various sample lysates using GTX66565 IST1 antibody. Dilution : 1:3000 Loading : 25microg per lane
Product group Antibodies
GeneTex
IST1 antibodyGTX66565
ApplicationsWestern Blot
TargetIST1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-IST1 Antibody144-61474
ApplicationsWestern Blot
TargetIST1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-IST1/OLC1 Antibody, Affinity PurifiedA305-411A
ApplicationsImmunoPrecipitation, Western Blot
TargetIST1
  • SizePrice
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Product group Antibodies
Atlas Antibodies
Anti-IST1 AntibodyHPA054532
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetIST1
  • SizePrice
Figure 1. Western blot analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MRC-5 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1/IST1 antigen affinity purified polyclonal antibody (Catalog # A05507-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1/IST1 at approximately 40 kDa. The expected band size for HIST1/IST1 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-hIST1/IST1 Antibody Picoband(r)A05507-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIST1
  • SizePrice
Product group Antibodies
Assay Genie
IST1 AntibodyCAB9401
TargetIST1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IST1 AntibodyLS-C830187
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetIST1
  • SizePrice