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ISYNA1 Rabbit pAb

A8965
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetISYNA1
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Overview

  • Supplier
    ABclonal
  • Product Name
    ISYNA1 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51477
  • Target name
    ISYNA1
  • Target description
    inositol-3-phosphate synthase 1
  • Target synonyms
    INO1, INOS, IPS, IPS 1, IPS-1, inositol-3-phosphate synthase 1, MI-1-P synthase, MIP synthase, myo-inositol 1-phosphate synthase A1, testis secretory sperm-binding protein Li 200a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPH2
  • Protein Name
    Inositol-3-phosphate synthase 1
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ISYNA1 using anti-ISYNA1 antibody (A08693-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISYNA1 antigen affinity purified polyclonal antibody (Catalog # A08693-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ISYNA1 at approximately 61 kDa. The expected band size for ISYNA1 is at 61 kDa.
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