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ProductsBack/ITGA2B / CD41 Antibody (clone MWReg30, FITC)

ITGA2B / CD41 Antibody (clone MWReg30, FITC)

LS-C811413
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGA2B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA2B / CD41 Antibody (clone MWReg30, FITC)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MWReg30
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID3674
  • Target name
    ITGA2B
  • Target description
    integrin subunit alpha 2b
  • Target synonyms
    alphaIIb protein; BDPLT16; BDPLT2; CD41; CD41B; GP2B; GPalpha IIb; GPIIb; GT; GT1; GTA; HPA3; integrin alpha-IIb; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41); platelet fibrinogen receptor, alpha subunit; platelet glycoprotein IIb of IIb/IIIa complex; platelet membrane glycoprotein IIb; platelet-specific antigen BAK; PPP1R93; protein phosphatase 1, regulatory subunit 93
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD41/Integrin Alpha 2B/ITGA2B antigen affinity purified polyclonal antibody (Catalog # A01102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD41/Integrin Alpha 2B/ITGA2B at approximately 125 kDa. The expected band size for CD41/Integrin Alpha 2B/ITGA2B is at 113 kDa.
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