ITGA4-ITGB7: MAdCAM-1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

BPS Bioscience

ITGA4-ITGB7: MAdCAM-1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

23

Neutralisation/Blocking

Research Use Only

Biotin

Integrin alpha-4

The ITGA4-ITGB7: MAdCAM-1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between ITGA4-ITGB7 (Human Integrin alpha 4 beta 7 or alpha4beta7) and MAdCAM-1 (Mucosal addressin cell adhesion molecule-1) for screening and profiling applications. This kit comes with enough purified recombinant ITGA4-ITGB7 (amino acids 34-977 (ITGA4) & 20-723 (ITGB7)), biotinylated MAdCAM-1 (amino acids 19-333), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. ITGA4-ITGB7: MAdCAM-1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with ITGA4-ITGB7 protein. After coating and blocking, biotinylated MAdCAM-1 is added in an optimized assay buffer. Next, unbound biotinylated MAdCAM-1 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of MAdCAM-1 binding to ITGA4-ITGB7. Need us to run inhibitor screens or profile your compounds against ITGA4-ITGB7: MAdCAM-1 ? Check out our Immunotherapy Biochemical Screening Services.

41116100

Human