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ProductsBack/ITGA9 / Integrin Alpha 9 Antibody

ITGA9 / Integrin Alpha 9 Antibody

LS-C749020
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetITGA9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA9 / Integrin Alpha 9 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 114kDa, while the observed MW by Western blot was 114kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 114kDa, while the observed MW by Western blot was 114kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3680
  • Target name
    ITGA9
  • Target description
    integrin subunit alpha 9
  • Target synonyms
    ALPHA-RLC, ITGA4L, RLC, integrin alpha-9, integrin alpha-RLC
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 9 antibody [N1N2], N-term (GTX102728) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of Integrin Alpha-9/ITGA9 using anti-Integrin Alpha-9/ITGA9 antibody (A06851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha-9/ITGA9 antigen affinity purified polyclonal antibody (Catalog # A06851-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha-9/ITGA9 at approximately 120 kDa. The expected band size for Integrin Alpha-9/ITGA9 is at 115 kDa.
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