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ProductsBack/ITGAM / CD11b Antibody (clone MEM-170, Azide-free)

ITGAM / CD11b Antibody (clone MEM-170, Azide-free)

LS-C45727
LifeSpan BioSciences
ApplicationsFunctional Assay, Flow Cytometry
Product group Antibodies
TargetITGAM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGAM / CD11b Antibody (clone MEM-170, Azide-free)
  • Delivery Days Customer
    23
  • Applications
    Functional Assay, Flow Cytometry
  • Applications Supplier
    Flo, Func
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MEM-170
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3684
  • Target name
    ITGAM
  • Target description
    integrin subunit alpha M
  • Target synonyms
    antigen CD11b (p170); CD11 antigen-like family member B; CD11B; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MO1A; neutrophil adherence receptor alpha-M subunit; SLEB6
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # A00144-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD.
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