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Western Blot analysis of HepG2 cells using Integrin beta4 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Integrin beta4 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Integrin beta4 Polyclonal Antibody

ITGB4 Antibody

CSB-PA003051
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetITGB4
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITGB4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3691
  • Target name
    ITGB4
  • Target description
    integrin subunit beta 4
  • Target synonyms
    CD104; CD104 antigen; GP150; integrin beta-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16144
  • Protein Name
    Integrin beta-4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (A01015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat ovary tissue lysates, Lane 8: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # A01015-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 202KD.
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