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WB analysis of various sample lysates using GTX66117 ITPR3 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66117 ITPR3 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66117 ITPR3 antibody. Dilution : 1:3000 Loading : 25microg per lane

ITPR3 antibody

GTX66117
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetITPR3
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Overview

  • Supplier
    GeneTex
  • Product Name
    ITPR3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3710
  • Target name
    ITPR3
  • Target description
    inositol 1,4,5-trisphosphate receptor type 3
  • Target synonyms
    CMT1J, IMD132, IMD133, IP3R, IP3R-3, IP3R3, inositol 1,4,5-trisphosphate-gated calcium channel ITPR3, IP3 receptor, inositol 1,4,5-triphosphate receptor, type 3, insP3R3, type 3 InsP3 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14573
  • Protein Name
    Inositol 1,4,5-trisphosphate-gated calcium channel ITPR3
  • Scientific Description
    This gene encodes a receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. The receptor contains a calcium channel at the C-terminus and the ligand-binding site at the N-terminus. Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth. [provided by RefSeq, Aug 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITPR3 using anti-ITPR3 antibody (PB9428). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPR3 antigen affinity purified polyclonal antibody (Catalog # PB9428) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPR3 at approximately 270-300 kDa. The expected band size for ITPR3 is at 304 kDa.
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