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ITPRIP Polyclonal Antibody

RD80694A
Reddot Biotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetITPRIP
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    ITPRIP Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID85450
  • Target name
    ITPRIP
  • Target description
    inositol 1,4,5-trisphosphate receptor interacting protein
  • Target synonyms
    D1A, DANGER, KIAA1754, bA127L20, bA127L20.2, inositol 1,4,5-trisphosphate receptor-interacting protein, DANGER family member 1A, inositol 1,4,5-triphosphate receptor-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a membrane-associated protein that binds the inositol 1,4,5-trisphosphate receptor (ITPR). The encoded protein enhances the sensitivity of ITPR to intracellular calcium signaling. Alternative splicing results in multiple transcript variants.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITPRIP using anti-ITPRIP antibody (A11503-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissues lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPRIP antigen affinity purified polyclonal antibody (Catalog # A11503-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPRIP at approximately 80 kDa. The expected band size for ITPRIP is at 62 kDa.
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