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JADE2 / PHF15 Antibody (N-Terminus)

LS-C47105
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetJADE2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    JADE2 / PHF15 Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1 µg/ml), WB (1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23338
  • Target name
    JADE2
  • Target description
    jade family PHD finger 2
  • Target synonyms
    JADE-2, PHF15, E3 ubiquitin-protein ligase Jade-2, PHD finger protein 15, jade family PHD finger protein 2
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF15/JADE2 using anti-PHF15/JADE2 antibody (A12993-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF15/JADE2 antigen affinity purified polyclonal antibody (Catalog # A12993-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF15/JADE2 at approximately 120 kDa. The expected band size for PHF15/JADE2 is at 87 kDa.
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